Materials List
Affordable and Accessible

Don't worry if the instruments in our video look specialized, you can easily substitute these with common household items, which are both affordable and readily available.

For example, instead of using a laboratory beaker, any kitchen measuring cup will do. Likewise, a clean syringe can serve as an effective alternative to a pipette.

It’s crucial that all items used are impeccably clean and disinfected with alcohol to prevent any contamination.

This ensures that you can conduct your experiments safely and effectively without the need for specialized equipment.

Things you will need.

Colchicine While botanical-grade colchicine is available as a direct-use powder, we opt for the pharmacy-grade colchicine, typically used to treat gout.

This form is more readily accessible and comes in 1mg tablets. It's important to note that either type can be used, but the pharmacy-grade tablets are often easier to find and handle for our purposes.

You can buy it at the pharmacy, it is a remedy to treat Gout, we use the 1mg version, with 20 tablets you can mutate 100 seeds or several plants.

Pipette You can use a syringe (without the needle) or the dropper of some medicine from the pharmacy.

Measuring glass Instead of a 50ml beaker, use any kitchen measuring cup that includes a 50ml mark. Alternatively, you can measure 50ml using three and a third tablespoons (15ml each).

Petri dish Use a shallow container with a lid, such as one for germinating seeds. Ensure it’s clean and flat to effectively hold your seeds

Medicinal cotton The one that is sold at the pharmacy (a small amount).

Disposable gloves you can buy them at any pharmacy.

Paper napkins or some paper towels.

100 ml of water (Chlorine free) It can be distilled, mineral. But the most important thing is that it does NOT have chlorine.

Plant Material Choose between germinated seeds showing a radicle (the little white tail), a rooted clone, or a vegetative branch if you decide to mutate a specific part of a plant.

Now that we have our materials list. The next step is to make the colchicine solution and later mutate our first plant and create our polyploid.

Are you ready?

Make the Colchicine Solution

Step-by-Step Guide to Preparing a Colchicine Solution

How to mutate from seedlings

This means that the entire plant, from the roots to the leaves and eventual flowers, will exhibit the effects of the mutation

How to mutate from Apical Buds

Inducing mutations in the apical buds of a branch affects only the new growth from that bud.
This method allows specific branches to be targeted for mutation, which can be useful for experimental and selective breeding purposes